The molluscan assemblages occurring in profiles of calcareous tufa in the Pieniny Mountains (the Carpathians, Southern Poland) were subject to a detailed malacological analysis. The study included 27 sites of these deposits. Three of them represent the Early and Middle Holocene, and the remaining is tufa deposited in the Subatlantic Phase, mainly in the historical period. The conducted analyses enabled recognition of several types of calcareous tufa formed under different morphological conditions and containing various molluscan assemblages. On the basis of the gathered material, defining nine types of malacocenoses was possible. Each of them strictly corresponds to the conditions prevailing in time and place of deposits deposition and represents a specified climatic phase of the Holocene. The observed sequences of molluscan assemblags were the basis for paleoenvironmental reconstruction, determining the main phases of climatic changes and the human impact on the natural environment. The results of malacological analysis were complemented with determination of the age of deposits by means of radiocarbon dating and the results of palynological analyses conducted in the sites of peatbogs occurring in Podhale. The carried out reconstruction indicates similarities and differences in the paleogeographic development of mountain and upland areas of Central Europe.